Fluorescent treponemal antibody-absorption double-staining procedure
نویسندگان
چکیده
منابع مشابه
Fluorescent treponemal antibody absorption double-staining test evaluation.
The fluorescent treponemal antibody absorption (FTA-ABS) double-staining (DS) test has been developed for microscopes equipped with incident illumination, and the procedure offers many advantages over the FTA-ABS test when tests are performed with this equipment. In this study, 346 fresh sera, including 35 from patients with syphilis, were evaluated by the FTA-ABS DS test. Parameters for invest...
متن کاملDouble-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test.
The fluorescent treponemal antibody absorption (FTA-ABS) double-staining procedure was reproducible, comparable to the conventional test, and easy to read. We recommend the use of the FTA-ABS double-staining procedure for microscopes with incident illumination, the 100 x/1.30 oil achromatic objective and the 6.3 x ocular to obtain optimal fluorescence, and the KP560 as a barrier filter to exclu...
متن کاملFluorescent treponemal antibody studies.
Fluorescent antibody methods were originally introduced by Coons and associates in 1942. Included in this first publication was a procedure for antibody-fluorescein conjugation and a description of fluorescent reactions obtaincd by means of ultraviolet light microscopy. This communication, and later ones by other authors, indicated that fluorescent antibody procedures were practical for rapid a...
متن کاملShelf life of fluorescent treponemal antibody-absorption test reagents.
Properly prepared, standarized, and stored fluorescent treponemal antibody-absorption (FTA-ABS) reagents have been shown to have stabilities equal to other biological reagents. A liquid antigen over 10 years old has been shown to give a satisfactory reaction. Newer preparations have now been shown to be stable for over 5 years, and the tests on each are being continued. The very new liquid anti...
متن کاملFluorescent treponemal antibody absorption (FTA-ABS) test in yaws.
The pathogenic treponemes, T. pertenue and T. pallidum, produce similar antibodies in the serum of a person infected with either organism. Thus sera from patients with yaws react similarly to sera from syphilitic patients in the conventional tests for syphilis and in the Treponema pallidum immobilization (TPI) test. The most recent test developed for the detection of syphilis is the fluorescent...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1981
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.14.2.184-188.1981